Journal article
Protection by methylproamine of irradiated human keratinocytes correlates with reduction of DNA damage
PN Lobachevsky, RS Vasireddy, S Broadhurst, CN Sprung, TC Karagiannis, AJ Smith, IR Radford, MJ MacKay, RF Martin
International Journal of Radiation Biology | TAYLOR & FRANCIS LTD | Published : 2011
Abstract
Purpose: The therapeutic ratio for ionising radiation treatment of tumour is a trade-off between normal tissue side-effects and tumour control. Application of a radioprotector to normal tissue can reduce side-effects. Here we study the effects of a new radioprotector on the cellular response to radiation. Methylproamine is a DNA-binding radioprotector which, on the basis of published pulse radiolysis studies, acts by repair of transient radiation-induced oxidative species on DNA. To substantiate this hypothesis, we studied protection by methylproamine at both clonogenic survival and radiation-induced DNA damage, assessed by γH2AX (histone 2AX phosphorylation at serine 139) focus formation en..
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Awarded by National Breast Cancer Foundation
Funding Acknowledgements
This research was supported by Australian National Health and Medical Research Council Project Grants No. 145780, No. 288713 and No. 454674, the National Breast Cancer Foundation Grant (Australia) No. PG-08-06 and a L.E.W. Carty Charitable Fund Grant No. 6392.